Microscopy Community

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Examples of microscopy infrastructures at Lund University

Fluorescence microscopes and live cell imaging for microbiology at Dept. of Biology. Zeiss AxioImager.Z2 and AxioObserver.Z1 instruments, Hamamatsu sCMOS and EM CCD cameras, and CellASIC ONIX microfluidic cell perfusion system. Contact Klas.Flardh@biol.lu.se for more info, or visit the MBio web site.

Live Cell Imaging Platform at MultiParkNikon Ti-E is a fully motorised research microscope with Spectra X light engine and EMCCD, suitable for imaging of fluorescence labelling, such as DAPI, CFP, GFP, YFP, mCherry and Cy5. For more information contact Jia-Yi.Li@med.lu.se

Confocal Microscope Platform at MultiParkLeica SP8 confocal microscope for imaging of fixed tissue and cells. This inverted confocal microscope includes a motorized stage and a two channel confocal scanning head with spectral detection. The system have 3 LED lasers with these laser lines: 405, 488, 514, 552 and 638 nm and two detectors of which one is a standard detector and one is a HyD SP detector. For more information contact Bengt.Mattsson@med.lu.se

Plate Runner Platform at MultiParkHigh content screening (HCS) / Analysis (HCA) enables automated acquisition, visualization and quantification of a large number of images such as rapid snapshot acquisition of fluorescence images of cells and small organisms. For more information contact Anna.Hammarberg@med.lu.se  

Cellomics Platform at MultiParkThe Cellomics ArrayScan VTI HCS Reader enables automated acquisition, visualization and quantification of a large number of images, with an automated fluorescence and brightfield microscopy allowing extraction of quantitative data from intensity and area measurement in high-throughput format. For more information contact Anna.Hammarberg@med.lu.se  

Image Analysis Software at MultiParkImaris version 8.4.1 Single-Full SF. MultiPark also has Arivis software. For more information contact gunnar.gouras@med.lu.se 

STED microscopy lab at Division of Solid State Physics / Department of Physics

STED microscope from Abberior Instruments combined with FLIM and polarization. 2D and 3D STED. Capable of two-color STED microscopy with lateral resolution ~50nm with excitation wavelengths 561nm and 640nm. STED laser wavelength 775nm. For more information contact jonas.tegenfeldt@ftf.lth.se 

Preclinical IVIS SpectrumCT 3D imaging for longitudinal studies

The IVIS Spectrum CT enables longitudinal workflows to characterize disease progression and therapeutic effect in animals throughout the complete experimental time frame with both quantitative CT and optical reconstructions. This instrument is the most sensitive detection technology ideal for: (I) 2D and 3D quantitative bioluminescence and fluorescence imaging (II) Cerenkov imaging for optical radiotracer imaging (III) Low dose and ultra fast microCT (IV) DyCE™ dynamic enhanced imaging for real time distribution studies of fluorochromes or PET tracers ideal for PK/PD applications

Contact: Ramin.Massoumi@med.lu.se, For scientific questions, please contact: wondossen.sime@med.lu.se

Nikon SIM-E Microscope, Nikon Ti2 Pathogen Live Microscope and Nikon TE-300 Microscope at IKVL, Nordenfelt Lab (Quantitative infection biology).

The SIM-E is a fluorescence super-resolution microscope,  the Ti2 is a fluorescence microscope for live imaging of pathogens, BSL2-compliant and the TE-300 is a fluorescence wide-field microscope.

For more information contact pontus.nordenfelt@med.lu.se

Facilities for 3D optical imaging and electron microscopy at Materials Engineering with instruments including E-SEM FEI Quanta 200 MKII with analytical system Pegasus (EBSD+EDS) from EDAX and optical microscope Keyence VHX-6000.

For more info visit the homepage or contact Dmytro Orlov, dmytro.orlov@material.lth.se 

SamplePrep - Facility for TEM and site-specific sample preparation from hard materials 

The infrastructure offers a complete set of tools for the preparation of site specific and transmission electron microscopy (TEM) specimens from hard solid materials with the accuracy of target positioning at least 10 μm.

More info here

IVIS-CT at Medicon Village, contact Ramin Massoumi Molecular Tumor Pathology, ramin.massoumi@med.lu.se

The TMA (Tissue Micro Array) centre is located at the Pathology building at Skåne University Hospital in Malmö

Here you get professional preparation of TMAs as well as other services such as embedding, sectioning, routine histological stainings and IHC.

More info visit this link

Scanning tunneling microscopes and atomic force microscopes

The division of synchrotron radiation research hosts one of Sweden’s largest facilities for scanning probe microscopy, including six scanning tunneling microscopes and two atomic force microscopes.

A more detailed description can be found via this link: www.sljus.lu.se/spm

VEVO MD IMAGING SYSTEM - an Ultra High Frequency ultrasound system at Pediatrics

Use the VEVO imaging system to get the highest resolution possible in medical ultrasound when imaging the first 3 cm of the body.

Fore more information contact  pia.tham@med.lu.se

Alicona InfiniteFocus microscope - 3D surface reconstructions for detailed observations of shape, surface roughness and 3D measurements at LTH

The Alicona InfiniteFocus microscope is suitable for objects as large as 30cm x 30cm and as small as anything that can be resolved by visible light. Suitable microscope parameters can be set automatically, which allows multiple users in different fields with varying level of expertise to use the system independently.

Contact:

Filip Lenrick (filip.lenrick@iprod.lth.se)

Jakob Johansson (jakob.johansson@iprod.lth.se)

SM-ELYRA - Superresolution microscope at CRC 

With superresolution structured illumination (SR-SIM) you image fine structural details while remaining free to label your samples with conventional dyes. With superresolution photoactivated localization microscopy (PALM) you can study endogenously-expressed photo-switchable fluorescent proteins.

For more information contact

Erik.renstrom@med.lu.se or Enming.zhang@med.lu.se

LBIC – Lund University BioImaging Centre, Medical faculty (e.g., light microscopy, electron microscopy)

http://www.med.lu.se/bioimaging_center

affiliated is the Preclinical MSOT (Multispectral Optoacoustic Tomography) at MV, Animal house. 

contact Chris D. Madsen, chris.madsen@med.lu.se

MBio – Microscopy unit at Biology, Science faculty (light and electron microscopy)

http://www.biology.lu.se/services/microscopy/electron-microscopy 

nCHREM – National Center for High Resolution Electron Microscopy, Engineering faculty (cryo electron microscopy and electron tomography) 

http://www.kilu.lu.se/cas/instruments/electron-microscopy/

4D Imaging Lab – Division of Solid Mechanics, Engineering faculty (x-ray tomography)

http://www.solid.lth.se/resources/4d-imaging-lab/

Image facility at Immunology - competence and instruments for live cell imaging, studies of cell-cell interactions and more!

Read more about Imaging at Immunology

Two-photon microscopy at BMC - Instruments for in vivo and ex vivo studies!

Read more about two-photon microscopy at BMC

Optical projection tomography (OPT) 

Do you want high-resolution 3D images or map the distribution of a flourescent protein/molecule of interest? Use the OPT platform

Anterior chamber of the eye (ACE) platform

Direct visualization in a variety of tissues transplanted into the anterior chamber of the eye (ACE) provides a platform to screen drugs by performing long-term follow up and monitoring in target tissues. For access to competence and instruments please contact the ACE platform

Correlative Light and Electron Microscope (CLEM) 

CLEM allows the simultaneous analysis of specimens by fluorescence and scanning electron microscopy. With this new technology, you can combine immunological and three-dimensional information within the same image. Read more: www.med.lu.se/clem

Zeiss 710 Live Cell Inverted Confocal Microscope

This microscope is equipped with motorized stage, heated incubator, controller regulating O2, CO2 and humidity. 

For more information contact chris.madsen@med.lu.se

Leica SP8 confocal microscope at MultiPark

This inverted confocal microscope includes a motorized stage and a two channel confocal scanning head with spectral detection. For more information click here

Additional related resources at Lund University

Mathematical Sciences

provide expertise in development of new mathematical methods for problems arising in imaging and computer vision

Read more and find contact information here and also here.

LUNARC

LUNARC is the center for scientific and technical computing at Lund University and provides computational resources within all aspects of computational science. Contact LUNARC to discuss your needs for image data handling, storage and processing!

Research Schools: Imaging of 3D structures, www.imagingresearch.lu.se, Advanced microscopy research school (ADMIRE), http://www.admire.lu.se/

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