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Proteins

   

With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design.

Below you can see some examples of the infrastructure for proteins, available for researchers at Lund University.

Biacore X100 - Surface Plasmon Resonance

The Biacore X100 instrument is open for external users to hire after completed course or we can provide a full service.

The Biacore X100 uses the surface plasmon resonance (SPR) technique to monitor molecular interactions in real time. The procedure is as follows; one of the interacting molecules (the ligand) is attached to a surface of a sensor chip, and the other interacting molecule (analyte) is injected and will flow over the sensor chip. If any binding occurs between the ligand and the analyte, the refractive index will change at the sensor surface, that is proportional to the change in mass concentration, and association and dissociation rates of the interaction may be determined.

Read more: Biacore X100- Surface Plasmon Resonance

biacore

BioMAX

BioMAX is the first X-ray macromolecular crystallography beamline of MAX IV Laboratory. It is a state-of-the-art resource accommodating multiple cutting edge experimental possibilities. The beamline experiment set-up is highly automated, in terms of both sample handling hardware and data analysis.

Due to its extensive energy tunability, BioMAX is an ideal source for de novo phasing using the anomalous signal of heavy elements. The small beam cross-section and parallel beam makes BioMAX suitable for X-ray crystallography using microcrystals and ultra large unit cells.

Read more: BioMAX at MAX IV

biomax
Electron density map of the cofactor NADP bound to FabG. Courtesy of Robert Schnell, Karolinska Institutet

LP3 - Lund Protein Production Platform

A cross-faculty facility for protein production, purification and crystallisation with:

  • Cloning - design of a vector construct
  • Protein production in bacterial cells and insect cells
  • Protein labelling
  • Protein purification using chromatography equipment
  • High-throughput protein crystallisation
  • Microbiological growth monitoring (Bioscreen C)

At LP3 we exchange scientific ideas for dissemination and assimilation of new methodologies for protein production, purification and analysis. We also offer research training and development of skills in experimental protein science for PhD students and postdocs.

Read more: LP3 - Lund Protein Production Platform

lp3

MAX IV

The MAX IV Laboratory serves 1000 users per year and deliver science in a broad range of fields including structure and dynamics of proteins in solution, protein crystallography and biomedical imaging. After almost 30 years of successful operation and research the old facility will be closed December 2015 to open the door for the new MAX IV facility.

Read more: MAX IV

Image of MAX IV exterior

MESO QuickPlex at MultiPark Platform

The MESO QuickPlex is a multiplexing plate reader using high-performance, electrochemiluminescence (ECL) immunoassays for detecting bio markers.

It is quick (90 seconds for one plate) and can handle many samples at the same time. The ECL system allows for detection with high sensitivity and wide dynamic range, which in combination with multi-array technology enables accurate measurements of multiple analytes in low sample volume. The instrument is easy to learn and use. There is more than 600 immunoassays for a range of applications including immunology, inflammation, oncology, neurobiology, and toxicology, in various assay formats.

Read more: MESO QuickPlex at MultiPark Platform

mesoquickplex

Thanks to our world class research infrastructure, Lund University is well equipped to help lead the way towards future scientific breakthroughs. Your imagination is the only limit to what can be done.

Watch a video about our infrastructure

Watch a video about our infrastructure

Watch a video about our infrastructure

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