Lund University Bioimaging Center

Faculty of Medicine | Lund University

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Advanced Light Microscopy

Nikon Confocal A1+ microscope

Fully-automated confocal imaging system capable of capturing high-quality confocal images of cells and molecular events at high speed and enhanced sensitivity.

Available laser lines: 405 nm, 488 nm, 561 nm, and 640 nm
Detectors: 2 GaAsP and 2 PMT detectors
Software: NIS-Elements
Booking time:  Please contact Sebastian Wasserstrom via email at Sebastian.Wasserstrom@med.lu.se

To find out more about the confocal technique go to: http://www.microscopyu.com/articles/confocal/confocalintrobasics.html

confocal
   

Nikon STORM microscope

The STochastic Optical Reconstruction Microscope (STORM) is a super resolution technique that uses technology licensed from Harvard University.  N-STORM provides dramatically enhanced resolution that is 10 times that of conventional optical microscopes.

Available laser lines: 405 nm, 488 nm, 561 nm, and 647 nm (high powered)
Camera: Andor iXon 897 EMCCD
Software: NIS-Elements
Booking time:  Please contact Sebastian Wasserstrom via email at Sebastian.Wasserstrom@med.lu.se

To find out more about the STORM technique go to: http://www.microscopyu.com/print/articles/superresolution/stormintro-print.html

storm
   

Nikon Live Cell Total Internal Reflectance Fluorescence (TIRF) Microscope

Nikon’s TIRF system is a fluorescence microscopy technique that gives superior signal to noise images than conventional widefield fluorescence. The increase in the signal to noise ratio in TIRF images allows imaging and tracking single molecules.  The system is equipped with an Andor Ultra 897 camera, which enables users to image up to 11,000 frames/second (in select modes).  The high speed of the camera allows for tracking of fast moving systems within a live cell.

a tubulin tirf
TIRF image

Incubator: Stage top incubator with temperature control

Available laser lines: 405 nm, 488 nm, 561 nm, and 640 nm

Camera: Andor Ultra 897 EMCCD with Optomask

Software: NIS-Elements

Booking time: Please contact Sebastian Wasserstrom via email at Sebastian.Wasserstrom@med.lu.se

To find out more about the TIRF technique go to http://www.microscopyu.com/articles/fluorescence/tirf/tirfintro.html

a tubulin epi
Widefield image

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